Objectives: To monitor of gene expression profile in human dental pulp ( HDP ) cells by enamel matrix derivative ( EMD ) treatment was examined to identify the gene using cDNA microarray method. Methods: HDP cells were obtained from the third molars extracted from healthy young patients during the course of orthodontic treatment. The patients were given informed consent prior to providing the sample. HDP cells were incubated with/without 50 mg/ml EMD for 3, 12, 24, 72 h, and mRNA were extracted, and mRNA was reverse-transcribed to cDNA with Cy3-dUTP or Cy5-dUTP. The fluorescent labeled samples were hybridized on a human cDNA microarray ( Clontech, 1,100 genes ), and the fluorescence intensity of each gene was measured using an array scanner. The data was analyzed using the Gene Spring genetic analysis system. Results: From the analytical results of gene expression fluctuation of pulp cell with EMD, many genes increased their mRNA levels including serum iducible kinase, cycline dependent kinase 2 and IGF-1. Conclusion: Identified genes found in this study may involved in cell proliferation and calcification process. Gene expression profiling using cDNA microarray shoud be useful understanding the biological effect of EMD to HDP cells.

This study was supported by a Grant from Ministry of Education, Culture, Sports, Science and Technology to promote 2001-Multidisciplinary Research Projects ( in 2001-2005 ) and a Grant-in-Aid for Scientific Research (C)(2) 14571827 (2002-2003).

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