| 2068 Measurement of Lactic Acid Production of Carious Bacteria in the Mouth: Reliability of the Novel Biochemical Diagnostic Device ClinproTM Cario L-PopTM | ||
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I. HAEBERLEIN, O. KAPPLER, A. SAFI, and R. GUGGENBERGER, 3M ESPE AG, Seefeld, Germany Although all individuals harbor carious bacteria in their oral-micro-flora the susceptibility of the individual for caries lesions is different. Clinical studies have shown that the correlation between the number of indicator bacteria and caries risk frequently varies. ClinproTM Cario L-PopTM CCLP)(3M ESPE AG) is a biochemical device which measures the lactic acid produced by carious bacteria in presence of digestible sugars using biofilm samples from tongue. The measured metabolic activity indicates the power of carious bacteria to promote the caries process. Objective: The reliability of the CCLP test has been investigated by the measurement of the caries potential in patient's oral-micro-flora. Method: CCLP is based upon enzymatic oxidation of lactic acid by lactate-dehydrogenase coupled to a cascade of redox-indicators which generates a blue color signal. Biofilm samples were collected from the tongue of 50 randomly selected patients and then analyzed according to the manufacturer's instructions for use. The results are evaluated using the enclosed, nine field color chart. Results: 10 patients repeated CCLP 5x in succession. The individual's color signals were reproduced 100%. 20 patients used CCLP in the morning and afternoon on three days in one week. The data obtained over 53 days was within the range of reliability according to the manufacturer's instruction for use. CCLP demonstrates a reliability of 88% no matter when it is applied during the day. 20 patients applied the device daily for 4 weeks. Among the 400 measurements 90.6% were within the range of reliability according to the nine field colour chart (6,3% showed a deviation of one colour field, 3.1% three colour fields). Conclusion: The reliability of CCLP to measure the power of carious bacteria residing in the patient's oral-micro-flora is promising. Further clinical studies considering different caries-risk-groups are required to evaluate the contribution of CCLP to improve the caries risk assessment. | ||
| Seq #214 - Caries Prediction, Clinical Trials, Diagnosis 3:45 PM-5:00 PM, Friday, 27 June 2003 Svenska Massan Exhibition Hall B | ||
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