| 0750 Anodic Oxidation and Hydrothermal Treatment of cpTitanium Surface Increases BMP-2 Expression in Adherent Macrophages | ||
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J. TAKEBE1, N. YOSHIHIRO1, C.M. CHAMPAGNE2, L.F. COOPER2, and K. ISHIBASHI1, 1 Iwate Medical University, School of Dentistry, Morioka, Japan, 2 University of North Carolina-Chapel Hill, School of Dentistry, USA Objectives: During the initial stages of endosseous implant wound healing, we hypothesize that resident macrophages within the blood clot play an important role in modulating the repair process and serving as a major source of osteoinductive cytokines. We previously reported that the murine macrophage cell line J774A.1 (Macrophage) expresses BMP-2 mRNA in an in vitro model of osseous wound healing, and that Macrophage BMP-2 mRNA expression on commercially pure Titanium (cpTi) implant substrates is influenced by the cpTi implant surface topography. In vitro and in vivo studies of implant osseointegration have shown the usefulness of a new implant coating method, that involves forming a thin hydroxyapatite (HA) layer on cpTi by anodic oxidation and hydrothermal treatment (HA/cpTi). The aim of this study was to investigate the cellular events of early wound healing on HA/cpTi endosseous implant material by measuring in vitro BMP-2 expression in Macrophage adherent to HA/cpTi implants. Methods: Macrophage were cultured on HA/cpTi implant and untreated cpTi implant substrates for a 24-72 hour period. The morphology of adherent Macrophage was examined by scanning electron microscopy (SEM), while Macrophage BMP-2 mRNA expression were measured by semi-quantitative RT-PCR. Macrophage BMP-2 protein expression was also examined by immunohistochemical staining. Results: Macrophage adhesion and spreading increased with time on both substrates, but was higher on HA/cpTi implant than on untreated cpTi implant. Relative BMP-2 over actin mRNA expression was higher on HA/cpTi than on untreated cpTi at 24h (0.348 versus 0) and at 72h (0.584 versus 0.189). The cytoplasm of adherent Macrophage was positive for BMP-2 by immunohistochemical staining. Conclusion: Macrophage BMP-2 expression is enhanced on HA/cpTi implant, and may be an important phenomenon in the process of implant osseointegration. Supported by a Grant-in-Aid for the Scientific Research (# 13771182) from the Japanese Ministry of Education, Science, Sports and Culture. | ||
| Seq #86 - Cell Response 1:45 PM-3:45 PM, Thursday, 26 June 2003 Svenska Massan H1 | ||
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