Residual caries following cavity preparation can contribute to restoration failure and the availability of a novel self-etching/priming system with potential antibacterial properties may improve restoration longevity. Objectives: to assess the in vitro antibacterial properties of a novel self-etching/priming system (ABF), containing antibacterial monomer, MDPB, on natural carious lesions. Methods: Cavities were prepared in extracted carious human teeth, such as to incompletely remove all carious dentine, and teeth allocated to five experimental groups (n=10 each group). Cavities were treated as follows, Group 1: ABF Primer and ABF Bond; Group 2: ABF Primer and Clearfil SE BOND-Bond; Group 3: Clearfil SE BOND-Primer and Bond; Group 4: AP-X-PLT; Group 5: no treatment. The tooth was then bisected through the cavity with a sterile rotary saw. One half of the tooth was fixed in formalin, processed for histology and sections Gram stained for bacterial detection. A 100 micron slice was cut from the other half of the tooth with a sterile rotary saw, prior to punch biopsy of the dentine around the cavity margins and in deeper tissue areas. Biopsy specimens were cultured aerobically in nutrient broth for 48 hours and the presence of viable bacteria assessed by turbidity. Results: Viable bacteria were detected in 3 out of 10 teeth (not from all areas of cavity margin) from each of Groups 1 and 2, in 7 out of 10 teeth in each of Groups 3 and 4 and in 10 out of 10 teeth in Group 5. Bacteria were detected in all tooth specimens from each of the groups by the Gram staining procedure. Technique controls with sound teeth showed no bacteria by either method. Conclusions: We conclude that cavity treatment with the ABF primer has antibacterial effects on any residual caries and may be beneficial in reducing secondary caries and restoration failure.

Back to the Dental Materials: VIII - Others-Non-metallic Program
Back to the 81st General Session of the International Association for Dental Research (June 25-28, 2003)

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