| 2247 Enamel Matrix Derivative Associated with Insulin-like Growth Factor-I on Periodontal Ligament Fibroblasts | ||
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D.B. PALIOTO1, R.D. COLETTA2, E. GRANER2, J.C. JOLY2, and A.F.M. LIMA2, 1 University of São Paulo - School of Dentistry of Ribeirão Preto, Brazil, 2 University of Campinas - School of Dentistry at Piracicaba, Brazil Background: Enamel matrix derivative (Emdogain) has been recently shown to promote periodontal regeneration in vivo. Insulin-like growth factor-I is a potent modulator of periodontal regeneration by stimulating cell proliferation and differentiation and synthesis of type I collagen. However, the biochemical effects of these factors on periodontal fibroblasts are not completely understood. Objectives: The aim of this study was to evaluate the effect of enamel matrix derivative (EMD), Insulin-like growth factor-I (IGF-I) and the combination of these factors on the proliferation, adhesion, migration and synthesis of type I collagen by periodontal ligament fibroblasts (PDL). Methods: The proliferation rate was measured by automated cell counting at 1, 3, 7 and 10 days and by immunohistochemical expression of proliferating cell nuclear antigen (PCNA) after 24 h. The cell adhesion was analyzed by a colorimetric assay and cell migration was measured in Boyden chambers. Type I collagen expression and production was determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: The results indicated that the proliferative rate of PDL fibroblasts was significantly stimulated by EMD and IGF-I in a dose- and time-dependent manner. EMD enhanced the proliferation from day 1 to 3 while the effect of IGF-I could only be observed after 7 days of culture. Furthermore, the association of EMD plus IGF-I has significantly enhanced the proliferation rate comparing to the drugs used isolated (P<0.05). EMD, IGF-I and the combination of both factors have no effect on migration and adhesion indices. Addition of EMD, IGF-I or both unaffected type I collagen expression and production. Conclusion: Our results showed that EMD, IGF-I and the combination speed up cells metabolisms by stimulating the proliferation of periodontal ligament fibroblasts but do not affected cellular adhesion, migration and expression and production of type I collagen. FAPESP grant # 99/04921-4 and 99/10767-8. | ||
| Seq #227 - Biologic Response Modifiers and Tissue Formation 3:45 PM-5:00 PM, Friday, 27 June 2003 Svenska Massan Exhibition Hall B | ||
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