| 2510 Expression of Different Aquaporin Isoforms During Human and Mouse Tooth Development | ||
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S. FELSZEGHY1, L. M"DIS1, P. NÉMETH2, G. NAGY2, T. ZELLES3, P. AGRE4, J. LAURIKKALA5, I. THESLEFF6, and S. NIELSEN7, 1 Medical and Health Science Centre, University of Debrecen, Hungary, 2 University of Pécs, Hungary, 3 Semmelweis University, Budapest, Hungary, 4 Johns Hopkins University, Baltimore, USA, 5 Institute of Biotechnology University of Helsinki, Finland, 6 Institute of Biotechnology, University of Helsinki, Finland, 7 University of Aarchus, Aarhus, Denmark Objectives: In our previous study on human tooth germs a time and tissue dependend HA deposition were observed, suggesting a stage dependent water transport during tooth development. The aquaporins (AQP) constitute a family of water channels. They have been found in various human and animal tissues. However, there are no data available about the expression pattern of these water channel molecules in dental structures. Methods: We were examining whether AQP 1-5, 9 proteins are expressed in developing human and mouse tooth germs by immunohistochemistry and if expressed the cellular localization was examined using antibodies to human AQP1, rat AQP1, rat AQP2, rat AQP3, human AQP4, human AQP5, rat AQP5 and rat AQP9. Results: At the early bell stage AQP1 was only immunolocalized on the endothelial cells of small vessels, but no labelling was observed in the other structures of the tooth primordia. AQP2, AQP3 and AQP9 reactivity were not observed in tooth germs. In contrast strong AQP4 and 5 expression was then in dental lamina, inner enamel epithelium, stratum intermedium, stellate reticulum and the outer enamel epithelium. Oral epithelium also showed AQP4 and 5 immunolabeling. When the matrices of the dental hard tissues started to develop, AQP4 and 5 immunostaining was detected in the odontoblasts and on their processes, as well as in the secretory ameloblast and their apical processes. No signal was detected in control sections, where either (1) the primary antibody was omitted or (2) nonspecific IgG was substituted for primary antibody. Conclusions: In conclusion, AQP4 and AQP5 were detected in most cells in which previous studies demonstrated either HA and/or its major receptor CD44. This coincidence may imply that AQP water channels are also necessary for providing the hydrated ECM as a microenviroment for different cells during tooth development. | ||
| Seq #259 - Post-doctoral Category 11:00 AM-12:15 PM, Saturday, 28 June 2003 Svenska Massan Exhibition Hall B | ||
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