Objective: Resinous materials have received considerable attention as root-end filling materials due to their sealing ability. However, little is known about their effects on activities of osteoblasts which play an important role in the healing of surgical lesions. The purpose of this study was to examine the influences of four root-end filling materials on osteoblast proliferation and function using MC3T3-E1 cell culture system. Materials & Methods: A Bis-GMA based resin composite (CLEARFIL APX, Kuraray; APX), a 4META-MMA resin (Superbond C&B, Sun Medical; SB), a zinc oxide eugenol cement (Super EBA, Bosworth; EBA), and a resin modified glass-ionomer cement (Fuji IONOMER Type II LC, GC; LC2) were tested. MC3T3-E1 cells were plated on each of set materials and cultured for 3, 7, 14, or 21 days. After each incubation period, cell attachment and proliferation were observed by scanning electron microscopy, and alkaline phosphatase (ALP) activity was measured. For the control, cells were cultured without any materials. Results: No differences in attachment, spreading and proliferation of MC3T3-E1 cells on the material surface were observed between the control and two resinous materials APX and SB. On the other hand, cells adhered and proliferated poorly on EBA and LC2, exhibiting rounded shape with little spreading. When cultured on APX or SB, ALP activity of MC3T3-E1 cells was not influenced until 7 days, and suppressed more than the control thereafter (p<0.05, ANOVA, Scheffe's F test). For EBA and LC2, ALP activity was significantly decreased (p<0.05) throughout the experimental period. Conclusion: MC3T3-E1 cells show more favorable response to APX and SB compared with EBA and LC2. The results indicate that resinous materials may be advantageous for periapical tissue healing when applied as root-end fillings. This study was supported by a Grant-in-Aid for Scientific Research (No.14370619) from the Japan Society for the Promotion of Science.

Back to the Dental Materials: VIII - Others-Non-metallic Program
Back to the 81st General Session of the International Association for Dental Research (June 25-28, 2003)

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