Objective: It has been reported that micro-computed tomography (MCT) is a noninvasive technique that possesses the ability to visualize morphological characteristics in a detailed and accurate manner without destruction of the tooth. However, the accuracy of the MCT was just qualitatively evaluated. The aim of this study was to evaluate quantitatively the accuracy of the MCT by comparing the values obtained from MCT with those recorded on the other methods. Methods: Six central incisors and six first molars having intact crowns and fully formed roots were used. The teeth were scanned using a desktop X-ray micro-CT scanner, and the images were reconstructed and measured. For the reference values, the teeth were measured with 1) direct measurement, 2) measurement using 3D scanner and 3) measurement in the photographs. Results: There were significantly high correlations between each method. The linear measurement in photographs were significantly overestimated compared with the measurement by the other three methods. The other comparisons of linear measurements were statistically indifferent. The volume measurement with MCT scanning were significantly underestimated compared with the 3D scanning measurement. Conclusions: We concluded that the micro-computed tomography is reliable method for linear measurement. Therefore it might be a useful device for measuring the distance and observing with the three-dimensional reconstruction of inside and outside of tooth. It can be also used for study of the change in inside and outside of tooth structure. For the voluminous measurement, there are some problems should be solved; such as improvement of linear accuracy, management of the air and the noise of the tooth.

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