| 1854 The First Bioactive, Degradable Membrane for Guided Bone Regeneration | ||
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F.E. WEBER1, H.G. SCHMÖKEL2, E. PIRHONEN3, R.E. JUNG4, and K.W. GRÄTZ1, 1 University Hospital, Zurich, Switzerland, 2 University Bern, Switzerland, 3 Inion OY, Tampere, Finland, 4 University of Zurich, School of Dentistry, Switzerland Background: The placement of an implant demands for sufficient bone, which can be generated by the guided bone regeneration (GBR) technique. The main function for a membrane in GBR is to generate a compartment where bone regeneration can occur undisturbed from other tissues. Regarding the necessity of a second surgery to remove the membrane, the replacement of non-resorbable by resorbable membranes would be desirable. A further improvement of the GBR technique could be achieved by a bioactive membrane capable to accelerate bone formation. Aim: Characterization of the bioactivity of the degradable InionGTR-membrane used in a GBR in vivo and in vitro model system. Methods: In vivo testing was performed in rabbits on non-critical size defects (6 mm) in the skull. For each condition the percentage of defect area filled with new bone of 8 Goldner stained middle sections was determined after 4 weeks. P values were calculated according the t-test for unpaired probes. In vitro tests based on alkaline phosphatase activity (ALP) were performed with MC3T3-E1 cells which resemble preosteoblastic cells. Results: Compared to untreated control defects (31±4%, N=17), the Osseoquest (Gore, USA) (61±5%; N=8, P<.001) and the InionGTR membrane (Inion OY, Finland) (79 ± 5%, N=8, P<.000) improved the bone healing significantly. Due to the fact that the InionGTR membrane performed significantly better than the Osseoquest membrane (N=8, P<.048) we determined the effect of the InionGTR membrane on preosteoblasts. Application of InionGTR membrane to MC3T3-E1 cells increased ALP activity concentration dependently. With 2 mg membrane ALP was 2,5±0.3 times (N=6, P<.000) the control level and the mineralization of MC3T3-E1 cells determined after 4 weeks by alizarin-red staining had increased 1,5±0.05 times (N=6, P<.001) Conclusions: InionGTR membrane is the first GBR-membrane of the third generation because it combines physical properties needed for GBR with biodegradability and bioactivity.
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| Seq #193 - Implant Histology and Bone Regeneration 1:45 PM-3:45 PM, Friday, 27 June 2003 Svenska Massan H1 | ||
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