Objectives: Perforation of a tooth structure resulting in communication of the pulp space with periodontium occasionally occurs during endodontic therapy. For the best prognosis, the perforation area must be sealed as soon as possible. Because these materials will be in direct contact with periodontal tissues, their cytotoxic potential must be evaluated before clinical use. The cytotoxicity of perforation repair materials must be investigated to ensure a safe biological response. Methods: The study was to evaluate the effect of resin-modified glass-ionomer cement, compomer and composite resin on human gingival fibroblasts. Using an explant technique with the consent of the patient cultured human gingival fibroblasts from crown lengthening surgery. The sample of resinous perforation repair materials (2x3.5x35mm) were extracted with 4 ml of Dulbecco's modified Eagle's medium (DMEM) without serum. Extraction was performed in an atmosphere of 5% CO₂ and 95% air at 37°C for 72 h. All extracts were filtered for sterilization until used for each assay. Cytotoxicity was judged by using an assay of tetrazolium bromide reduction. Results: Resin-modified glass-ionomer cement Fuji II LC (FLC), compomer Compoglass (CG) and composite resin Spectrum TPH (TPH) were cytotoxic to primary human gingival fibroblast cultures (p<0.05). They also significantly inhibited cell growth and proliferation. However, TPH alone had an inhibitory effect on cell attachment (p<0.05). It was found that TPH was the most cytotoxic repair material among those tested in all cultures. The cytotoxicity decreased in the order of TPH>FLC>CG. Conclusions: The data presented here suggest that the use of compomer and resin-modified glass ionomer cement as root perforation repair materials may initially react more favorably to gingival fibroblasts than the tested composite resin. Supported by NSC grant 90-2314-B-040-017. hfm0118@mercury.csmc.edu.tw

Back to the Dental Materials: VIII - Others-Non-metallic Program
Back to the IADR/AADR/CADR 80th General Session (March 6-9, 2002)

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