| 3999 Histological characterization of early bone morphogenetic protein-induced osteogenesis | ||
|
J. VEHOF1, H. TAKITA2, Y. KUBOKI2, P. SPAUWEN1, and J.A. JANSEN1, 1University Medical Center Nijmegen, Netherlands, 2Hokkaido University, Sapporo, Japan Objectives: For the reliable clinical application of bone morphogenetic protein (BMP)-induced bone formation, it is relevant that a significant amount of bone is formed within a reasonable time frame. We know that the initial BMP-induced bone formation can be influenced by the chemical composition and 3-D spatial configuration of the used carrier material. Therefore, we compared the osteoinductive properties of porous titanium (Ti) fiber mesh with a calcium phosphate (Ca-P)-coating (Ti-CaP), Insoluble Bone Matrix (IBM), Fibrous Glass Membrane (FGM) and Porous Particles of Hydroxy Apatite (PPHAP) loaded with rhBMP-2 in a rat ectopic assay model at short implantation periods. Methods: Twelve Ti-CaP, 12 IBM, 12 FGM and 12 PPHAP implants, loaded with rhBMP-2 (Yamanouchi Parmaceutical, Japan), were subcutaneously placed in 16 rats for 3, 5, 7, and 9 days. Results: Histological analysis of the specimens demonstrated that IBM and Ti-CaP had induced ectopic cartilage and bone formation by 5 and 7 days, respectively. On the other hand, in PPHAP bone formation and cartilage formation were seen together at 7 days. At 9 days, in Ti-CaP, IBM, and PPHAP, cartilage was seen together with trabecular bone. At 9 days, in FGM only cartilage was observed. The tissue response was also quantified according a gradient scoring system ranging between a score 0 (fibrous tissue) and a score 6 (bone). Quantitative rating demonstrated that bone formation proceeded significantly faster (P<0.05, t-test) in Ti-CaP and IBM than in FGM and PPHAP. FGM implants had at all time points a lower score than the other materials (P<0.05). Conclusions: We concluded that IBM, CaP-coated Ti mesh, FGM, and PPHAP provided with rhBMP-2 can indeed induce ectopic bone formation with a cartilaginous phase in a rat model at short implantation periods. Further, these findings suggest that cartilage cells are always present in rhBMP-2 induced osteogenesis. | ||
| Seq #371 - Bone Response to Surfaces/Scaffolds 3:45 PM-5:45 PM, Saturday, 9 March 2002 San Diego Convention Center Room 10 (Upper Level) | ||
|
Back to the Implantology Research Program
| ||