| 2034 Mutational analysis of hMSH2 gene in oral SCC | ||
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S. SANGUANSIN1, R. SURARIT1, W. WEERAPRADIST1, S. PETMITR2, and P. PUNYARIT3, 1Faculty of Dentistry, Mahidol University, Bangkok, Thailand, 2Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, 3Pramongkutklao medical college, Bangkok, Thailand Objective: The purpose of this study was to identify the mutation of hMSH2 DNA mismatch repair gene in Thai patients with oral squamous cell carcinoma. Methods: Oral cancer tissues were obtained from 32 patients with age ranged from 32-90 years old and 75% of these patients was betel quid chewers. The mutation of hMSH2 gene was determined using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and nucleotide sequencing. Results: The results showed no mutation of hMSH2 gene in the patients. Moreover changes in length of the poly (A) mononucleotide repeats BAT-26 and BAT-40 were also evaluated. 7 out of 32 cases exhibited a minor size variation in BAT-26. No variation of BAT-40 had been found. Conclusion: These experiments indicated that no evidence of mutation in hMSH2 DNA mismatch repair gene presented in Thai oral SCC. Supported by Faculty of Dentistry, Mahidol University and the Thailand Research Fund BR/05/2539. | ||
| Seq #190 - Non-imaging Diagnostics 11:00 AM-12:15 PM, Friday, 8 March 2002 San Diego Convention Center Exhibit Hall C | ||
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