Objective: To compare the response of cultured human gingival fibroblast (HGF) and periodontal ligament cell (PDLC) to two kinds of glass-ionomer cement (GIC) (Ketac Fil and Fuji II) in vitro with regards to understanding the biocompatibility of these materials and their suitability for use as subgingival restorative materials. Methods: Ketac Fil and Fuji II were mixed according to the manufacture's directions and condensed into tissue culture wells. PDLC and HGF were seeded into untreated tissue culture wells and GIC-treated tissue culture wells. Attachment activity and DNA synthesis of two kinds of cell were determined by measuring 3H-thymidine radioactivity. Results: No significant difference were observed between attachment levels and DNA synthesis for PDLC and HGF cultured on culture wells. But levels of attachment and DNA synthesis for HGF cultured on Ketac Fil and Fuji II were significantly higher than that for PDLC cultured on Ketac Fil and Fuji II. Conclusions: Ketac Fil and Fuji II may be acceptable subgibgival root surface restorative materials. But HGF cultured on Ketac Fil and Fuji II may be more growth easily . KEY WORDS£º Periodontal ligament cells; gingival fibroblasts; glass-ionomer cements; biocompatibility.

Back to the Dental Materials: VIII - Others-Non-metallic Program
Back to the IADR/AADR/CADR 80th General Session (March 6-9, 2002)

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