Objective: The aim of this study was using PCR for detection of Bacteroides forsythus and Porphyromonas gingivalis in subgingival plaque samples from 30 subjects to asses the relationship with distinct periodontal status. Methods: Thirty systemically healthy subjects were distributed in 4 groups, according their periodontal diagnosis: group 1- periodontal healthy (n=5); group 2- gingivitis (n=5); group 3- periodontitis with probing depth ≤5 mm (n=10); group 4- periodontitis with probing depth >5 mm (n=10). The individuals from group 2, 3 and 4 had at least 3 sites related with their diagnosis and one healthy site. The subgingival plaque samples were obtained using paper points inserted into periodontal pockets, 1 paper point each of the 3 sites that present periodontal deepest pockets; and 1 paper point into healthy gingival sulcus of the same subject. The samples were analyzed using PCR detection method. Results: Bacteria distribution detecting were different for healthy and disease sites. B. forsythus was not detected in any samples from group 1 and 2, and from the healthy sites of the others groups. This bacterium was detected in 70% and 100% in diseased sites from groups 3 and 4, respectively. P. gingivalis was detected in only one sample from healthy site of the group 4, and in the diseased sites of the groups 2, 3 and 4 the prevalence was 10%, 40% and 90%, respectively. Also, B. forsythus and P. gingivalis were detected at the same diseased site sample, in the groups 3 and 4, in 30% and 80%, respectively. Conclusion: These results can indicate an association between severity of periodontal disease and presence of B. forsythus and P. gingivalis . Supported by FAPESP grant # 99/09377-0

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