| 1085 Dentin formation following transdentinal delivery of bone morphogenetic protein beneath cavities restored using a liquid crystal resin-based material | ||
|
D.L. CARNES1, E.J. BOLAND1, C.B. HERMESCH1, R. JOUETT1, N. SATSANGI1, B.K. NORLING1, H.R. RAWLS1, S.T. WELLINGHOFF2, and M. MACDOUGALL1, 1University of Texas Health Science Center at San Antonio, USA, 2Southwest Research Institute, San Antonio, TX, USA Pulp tissue exhibits an intrinsic defense mechanism against insult or injury culminating in odontoblast differentiation and the deposition of reparative dentin. While these processes are an inherent response to injury they are not routinely evoked in modern clinical treatments. Members of the bone morphogenetic protein (BMP) family induce dentin formation in vivo when placed in contact with exposed pulp. Objectives: The purpose of this study was to examine dentin formation following transdentinal delivery of BMP beneath cavities restored using a liquid crystal resin-based material. Methods: Cavity preparations were created in the canines of ferrets using water-cooled burs under appropriate anesthesia. A solution of one part rhBMP-2 (R&D Systems, Minneapolis, MN) and 2 parts hydroxypropyl b cyclodextrin (Wacker-Chemie, Berghausen, Germany) (BMP final concentration=2.5µg / 7µl) was applied to the floor of the cavity in 1.5µl aliquots and air dried. The cavities were restored with a C6-t-butyl-diacrylate liquid crystal resin-based composite formulation developed by our group. The ferrets received intraperitoneal injections of fluorescent dye at 0, 20 and 35 days following cavity restoration to determine dentin formation rate. The ferrets were euthanised on day 40 and prepared for histological examination. Results: The dentin formation rate was 1µ / day in control canines (cyclodextrin vehicle alone) throughout the experimental period. BMP-treated canines demonstrated a 3-fold increase in dentin formation (3µ / day) in the area directly beneath the floor of the cavity during the first 35 days of the experimental period. The dentin formation rate was reduced during the final 5 days of the experimental period but remained elevated compared to control (2µ / day vs. 1µ / day). No inflammation was observed in the pulp. Conclusions: These data demonstrate that the BMP-cyclodextrin complex promotes immediate and sustained reparative dentin formation by transdentinal delivery mechanisms. Supported by NIH grant DE11688 | ||
| Seq #112 - Growth Factors; Regulation of Cell Function; Forensic Dentistry 3:45 PM-5:00 PM, Thursday, 7 March 2002 San Diego Convention Center Exhibit Hall C | ||
|
Back to the Pulp Biology Program
| ||