A hermetic apical sealing is fundamental for success of the root canal system obturation. Objectives: The purpose of this study was to evaluate "in vitro", the apical sealing ability of three endodontic cements. Methods: 36 human mandibular canines extracted were used. These were randomly divided into 03 experimental groups of 10 teeth each and into 02 positive and negative controls groups of 03 teeth each. The specimens were instrumented by Crown Down technique(until the 45 K-type file), and were obturated by lateral condensation technique(0,5 mm short of the apex), changing the sealer: G1-Pulp Canal Sealer EWT(Kerr); G2-Rickertxs cement(Lenzafarm); G3-Pulp Fill(Biodinâmica). The pulp chambers of all samples were filled with Ketac-Fil Plus(Espe Dental), and were kept in incubated to 37°C in chamber humidifier for 24 hours. The teeth were shielded, unless 03 mm at the apex and submerged in 2% Rodamina B dye for 48 hours. Then, the specimens were longitudinally sectioned and evaluate under surgical microscope(X20) 902 model(D.F. Vasconcelos), and dye penetration was measured in milimetric scale by 03 observers. The data were submitted the statistical analysis by ANOVA(F=0,78). Results: The evaluation showed that therexs no significant difference between the means of three tested groups. Conclusions: Based on the results obtained in this study, we conclude that Pulp Canal Sealer EWT, Rickertxs cement and Pulp Fill, showed the same apical sealing ability.

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