1255 Occluding effect of dentifrices on dentinal tubules
C.D. MICHELONI1, C.A.G. ARRAIS1, and M. GIANNINI2, 1State University of Campinas, Piracicaba, Brazil, 2STATE UNIVERSITY OF CAMPINAS, PIRACICABA, Brazil

The use of desensitizing dentifrices to occlude dentinal tubules consist of a treatment modality for hypersensitivity caused by exposed cervical dentin. Objectives: The aim of this study was to evaluate the dentin tubules occlusion ability of three commercially available dentifrices: Sensodyne (SE), Emoform (EM) and Sorriso (SO). Methods: Fifty freshly extracted human sound third molars were mesio-distally sectioned and cervical dentin was ground wet with 600,100,1200 SiC paper. Afterwards, they were polished with 6, 3, ½ and ¼ mm diamond pastes in order to simulate hypersensitive cervical regions. The samples were randomly divided into five groups (n=10): G1 - no brushing; G2 – brushing without dentifrice; G3 – brushing with SE; G4 – brushing with EM; G5 – brushing with SO. G3, G4, and G5 specimens were brushed with slurries of toothpastes at constant loading using the same reciprocal action eletric motor driven toothpaste machine (Equilabor), during 4 minutes per day, for 7 days. Specimens were prepared and inspected under SEM at X1,000 (DSM 940A/ZEISS) for calculation of the percentage of occluded tubules. Results: Data were statistically analyzed by ANOVA and Tukey test (p<0.05). All dentifrices promoted a high number of dentin tubules occlusion: G1- 37.31% ± 24.22%a; G2- 22.92% ± 15.19%a, G3- 80.12% ± 24.65%b, G4- 96.02% ± 5.24%b, G5- 98.83% ± 3.31%b. Conclusions: The use of dentifrices (G3, G4 and G5) promoted a statistically superior number of tubules occlusion than did the no brushing (G1) and brushing with no dentifrices (G2) groups.

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