Lipolysaccharide (LPS) is known to adhere to dental casting alloys, but its release from alloys has not been measured.  Objective:  To measure LPS released passively from dental casting alloys over time, and during subsequent ultrasonic treatment.  Methods:  Four alloys (2 high noble (HN) and 2 noble (N); 1cm diameter x 1mm thick; n=3) were polished, cleaned and disinfected, then exposed to LPS (E. coli, 100 mg/mL) for 24h.  Specimens were transferred into l mL phosphate buffered saline (PBS) for 24h, transferred to new PBS for 48h, then 96h.  Alloys were then placed into 1.0 mL of PBS and ultrasonically (Ult/Son) treated  for 10 min.  LPS concentrations (pg/mL) were assessed at each time interval and after Ult/Son treatment using the Limulus Amebocyte Lysate assay. PBS solution served as the control.  Results:  LPS release was significantly greater than controls (* in table, ANOVA/Tukey, a=0.05) for all alloys at 24h, but release was lower at other times (48, 96h). LPS release after Ult/Son treatment was significantly

LPS (pg/mL) (SD)	24h	48h	96h	PBS Control	Ult/Son Treatment
AuPt (HN)	870 (140)* a	39 (21)	24 (13)	22 (15)	60 (40) a
AuPd (HN)	440 (130) * b	130 (22) *	58 (33)	25 (13)	170 (70)* b
PdCuGa (N)	940 (160) * a	26 (13)	25  (13)	27 (13)	19 (13) a
PdAg (N)	410 (250) * b	70 (40) *	60 (27)*	23 (14)	140 (70)* b

greater than controls for the AuPd and PdAg alloys (*).  Alloy type significantly influenced LPS release at 24h and after Ult/Son treatment (a,b in table, ANOVA/Tukey). Conclusions: In this in vitro system, LPS release was dependent on the alloy type, and was greatest in the first 24h. (Support: Metalor and MCG Biocompatibility Prog.)

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