Objective: To date there has been very little data compared on the cytotoxic response of different cell lines to root canal sealers. The objective of this study was to determine the cytocompatibility of three different extracts of root canal sealers (AH plus, Canals and N2) and to compare the cytotoxic response of these materials on two different primary human oral fibroblasts (derived from gingiva and buccal mucosa) and one permanent hamster cell line (V79 cells) Methods: MTT colorimetric assay was developed to monitor mammalian cell survival and proliferation. Cytotoxicity was judged by tetrazolium bromide reduction. Results: The results showed that root canal sealers (AH plus, Canals and N2) were cytotoxic to primary human oral fibroblast cultures and V79 cells (p<0.05). It was found that N2 was the most toxic root canal sealer among those tested in all cultures. The toxicity decreased in an order of N2>AH plus=Canals. The sensitivity of toxicity depended on the materials tested and the cell culture system used. Conclusion: In vitro screening tests are very helpful to assay the biological effects of dental materials, but they may be limited in their ability to simulate the clinical condition. It is necessary to continue investigating endodontic materials until some are found that fulfill all the properties of ideal root canal sealers.

Back to the Dental Materials: VIII - Others-Non-metallic Program
Back to the IADR/AADR/CADR 80th General Session (March 6-9, 2002)

Top Level Search